Fig. 6
From: Inhibition of miR-542-3p augments autophagy to promote diabetic corneal wound healing
ATG4D is a functional target of miR-542-3p for regulating autophagy in diabetic trigeminal ganglion (TG) tissue. a Western blot analysis detected the expression of ATG4D proteins in TG tissue of control mice (Ctrl), diabetic mice (DM), and diabetic mice injected with miR-542-3p antagomir (DM + Ant-miR) (n = 3 per group). a1 Western blot bands of ATG4D protein. a2 Quantified intensities of Western blot bands for ATG4D compared with β-actin. b Immunofluorescence analysis detected the expression of ATG4D proteins in TG tissue of Ctrl, DM, and DM + Ant-miR (n = 3 per group). c Western blot analysis of LC3B, P62, ATG4D proteins in TG tissue of DM, DM + Ant-miR, diabetic mice injected with ATG4D ASO (DM + ATG4D ASO), and diabetic mice injected with miR-542-3p antagomir and ATG4D ASO (DM + Ant-miR + ATG4D ASO) (n = 3 per group). c1 Western blot bands of LC3B, P62, ATG4D proteins. Quantified intensities of Western blot bands for ATG4D (c2), LC3B (c3) and P62 (c4) compared with β-actin. d Immunofluorescence analysis showed the expression of ATG4D (d1), LC3B (d2) and P62 (d3) proteins in TG tissue of DM, DM + Ant-miR, DM + ATG4D ASO, and DM + Ant-miR + ATG4D ASO (n = 3 per group). Scale bar: 50 μm. ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001