Fig. 4
From: Inhibition of miR-542-3p augments autophagy to promote diabetic corneal wound healing
Potential autophagy-related miRNA-mRNA pairs in diabetic trigeminal ganglion (TG) tissue. Heatmap (a) and volcano plot (b) of DEmiRNAs. c Kyoto Encyclopedia of Genes and Genomes enrichment analysis of DEmiRNA target genes. d Autophagy-related miRNA-mRNA regulatory network analysis. e Quantitative real-time polymerase chain reaction validated the differential expression in TG tissue between control (Ctrl) and diabetic (DM) mice (n = 8). e1 Differential expression of miR-542-3p, miR-1968-5p, miR-3470a, miR-200a-5p, miR-200b-5p. e2 Differential expression of miR-669d-5p, miR-125a-5p, miR-7a-5p, miR-7b-5p, and miR-133b-3p. f Correlation analyses of miR-542-3p and ATG4D and miR-669d-5p and TBK1 (n = 16). g Potential binding site of miR-542-3p to the ATG4D 3' UTR and mutation site of the ATG4D-mut plasmid vector. h Dual-luciferase gene reporter assay for relative luciferase activity after cotransfection of ATG4D-wt and ATG4D-mut plasmid vectors with miR-542-3p mimics or negative control in HEK293T cells (n = 3 per group). i Western blot analysis showed ATG4D protein expression in TG tissue after subconjunctival injection with miR-542-3p agomir, agomir-NC, miR-542-3p antagomir, and antagomir-NC (n = 3 per group). i1 Quantified intensities of Western blot bands for ATG4D compared with β-actin. i2 Western blot bands of LC3B and P62 proteins. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001