Fig. 3
From: Inhibition of miR-542-3p augments autophagy to promote diabetic corneal wound healing
Enhanced autophagy promoted corneal debridement healing in type 1 diabetes mellitus (T1DM) mice. a Western blot analysis revealed expression of autophagy proteins in trigeminal ganglion (TG) tissue of control (Ctrl) mice, diabetic (DM) mice, RAPA-treated diabetic mice (DM + RAPA), and 3-MA-treated diabetic mice (DM + 3-MA) (n = 3 per group). a1 Western blot bands of LC3B and P62 proteins. Quantified intensities of Western blot bands for LC3B (a2) and P62 (a3) compared with β-actin. b Corneal whole-mount staining on day 5 in the Ctrl, DM, DM + RAPA and DM + 3-MA groups after debridement (n = 6 per group). b1 Fluorescent images of corneal nerve staining. b2 Peripheral corneal nerve density. c Corneal sensation levels of mice on day 5 of each group after debridement (n = 6 per group). d Corneas stained with fluorescein sodium of each group at 0, 12, 24, and 36 h after debridement (n = 6 per group). d1 Fluorescein-stained images of corneas. d2 Percentage of epithelial defect area. *, P < 0.05; **, P < 0.01; ****, P < 0.0001