Fig. 5
From: Homocysteine and mitochondrial quality control in diabetic retinopathy
Effect of diabetes on Cbs activity and homocysteine metabolism. a Cbs gene transcripts were analyzed in the retina from C57BL/6 J mice diabetic for 24 weeks by quantitative reverse transcription polymerase chain reaction using 18S rRNA as the housekeeping gene. Retina from Cbs+/+ and Cbs+/− mice diabetic for 24 weeks were analyzed for (b) Cbs activity using a fluorometric method, (c) H2S levels using N-dimethyl-p-phenylenediamine sulfate, and (d) total homocysteine levels via ELISA. Each measurement was performed in duplicate with 5–6 mice/group and results are presented as mean ± SD. Norm, normal Cbs+/+ mice; Diab, diabetes; Cbs+/+ and Cbs+/−, Cbs wildtype and heterozygous mice, respectively; *P < 0.05 vs. normal and #P < 0.05 vs. Cbs+/+ diabetic mice