Fig. 2
From: Homocysteine and mitochondrial quality control in diabetic retinopathy
Mitochondrial stress and homocysteine. Oxygen consumption rate (OCR) was measured in human retinal endothelial cells incubated in high glucose-homocysteine for (a) 24 h and (b) 96 h using a Seahorse XF Cell Mito Stress Test kit. Measurements were repeated two times, with each measurement containing eight wells/group; the results in the graphs are presented as mean ± SD. NG, 5 mM d-glucose; HG and HG/Hcy, 20 mM d-glucose without and with homocysteine, respectively; Hcy/GY, 20 mM d-glucose with homocysteine and GYY4137; l-Gl, 20 mM l-glucose; FCCP, carbonyl cyanide p-trifluoromethoxyphenylhydrazone; Rot/AA, rotenone/antimycin A