Fig. 5
From: Establishment of a novel ER-stress induced myopia model in mice
Scleral endoplasmic reticulum (ER) stress and apoptosis expression one week after treatment with tunicamycin (Tm) were detected by Western blotting and TUNEL assay, respectively. a Representative Western blotting results. b Densitometric analysis of blots in (a) in different groups. In each group of different Tm concentrations, n = 4 (each sample was a pool of three sclerae). In each group of different Tm applications, n = 3 (each sample was a pool of three sclerae). c TUNEL images (green: TUNEL, blue: DAPI) of sclerae in C57BL/6 J mice. Arrowheads indicate TUNEL-positive nuclei. Scale bar: 50 µm. Statistically significant differences are shown [*P < 0.05, one-way ANOVA with least significant difference (LSD) post hoc test]