Fig. 4
From: Establishment of a novel ER-stress induced myopia model in mice
Organization of scleral collagen visualized under a microscope by picrosirius red staining. a Scleral collagen visualized under bright field and polarized light. b Organization of scleral collagen after varying number of tunicamycin (Tm) applications. c Concentration of collagen bundles in the sclera after different number of applications of Tm (the collagen volume fraction: ratio of collagen bundles to total collagen, n = 5 eyes/group, mice with missing data are excluded). Statistically significant differences are shown [compared with control group, *P < 0.05, **P < 0.01, one-way ANOVA with least significant difference (LSD) post hoc test]