Fig. 6

Changes in retinal histology revealed in db/db mice. a, b Representative confocal images of the retinal sections of the two groups of mice processed using 4′,6-diamidino-2-phenylindole (DAPI, white or blue) and G Protein Subunit Alpha Transducin 2 (GNAT2, green). White scale bar: 20 μm. c–f Bar charts comparing the thickness of the outer nuclear layer (ONL) and inner nuclear layer (INL), the number of photoreceptors (DAPI stained nuclei in the ONL), and the number of cones (soma co-labelled by GNAT2) of db/+ mice (n = 7) and db/db mice (n = 6). (Data presented as mean ± SEM. Independent sample t-test: *P < 0.05, **P < 0.01, ***P < 0.001.) Representative confocal images of the retina sections of db/+ mice and db/db mice processed using PKCα (red) and DAPI (blue). White scale bar: 20 μm. h, i Bar charts comparing the rod bipolar cell densities and number of rod bipolar cell axon terminals of db/+ mice (n = 7) and db/db mice (n = 6). j Representative confocal images of retinal sections of db/+ mice and db/db mice stained with PKCα (red) and bassoon antibodies (green). White scale bar: 10 μm. k Bar charts comparing the rod bipolar cell dendritic boutons of db/+ mice (n = 7) and db/db mice (n = 5). Data presented as mean ± SEM. Independent sample t-test: ***P < 0.001