Fig. 5

Longitudinal profiles of retinal thickness measured with OCT revealed thinner retinas in db/db mice. a Line charts showing the mean total retinal thickness of db/+ mice (n = 14) and db/db mice (n = 11) at different experimental timepoints. b A b-scan tomogram from an equatorial slice of the scan showing the different sublayers in db/+ mice. c Representative OCT images showing a retinal segment approximately 118 μm away from the center of optic nerve head of db/+ and db/db mice at 25 weeks of age. Line charts showing the mean thickness of the d inner retina, e middle retina, and f outer retina of the two groups of mice at different experimental timepoints. Data presented as mean ± SEM. Simple main effect analysis: **P < 0.01, ***P < 0.001 vs. db/db mice; ^##P < 0.01, ^###P < 0.001 vs. db/db at 9 weeks. RNFL, retinal nerve fiber layer; GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; ELM, external limiting membrane; OS, outer segment; IS, inner segment; PR, photoreceptor; RPE, retinal pigmented epithelium