Fig. 2

Confluent cultures of primary RPE cells and primary CECs taken from non-pigmented rabbit eyeball. a Cell morphology of Rb-CEC and Rb-RPE at passages 0–2 were assessed with an inverted phase-contrast microscope (scale bar: 100 μm). b Representative immunofluorescence staining images of corneal endothelial markers ZO1 (green), ATP1A1 (red), retinal pigment epithelial markers MITF (green), and RPE65 (red) in Rb-RPE and Rb-CEC. Nuclei were stained with DAPI (blue) (scale bar: 50 μm). c Cell permeabilities of Rb-RPE and Rb-CEC using HRP tracer at 5 min, 10 min, 15 min, and 30 min, respectively. d The Na + /K + -ATPase activities of Rb-RPE and Rb-CEC at passage 2. Data are mean ± SEM. All results were obtained from three independent experiments. Significance (**P < 0.01, ns: nonsignificant) relative to Rb-CEC. RPE, retinal pigment epithelium; CEC, corneal endothelial cell; Rb-CEC: rabbit CECs; Rb-RPE, rabbit RPE cells; SEM, standard error of the mean