Fig. 2

Morphological changes of different regions of eyeballs. a Typical T1-weighted images (TIWI), typical T2-weighted images (T2WI), and representative 3-dimensional (3D) MRI scan in all three groups (n = 4). Scar bar = 1 mm. The right eye of six adult rats after 28 days of operation. The measured data of axial length (AL) by MRI was 53.26, 53.74, and 58.05 pixels in the control, sclerosant injection (SI), and circumlimbal suture (CS) groups, respectively; b Comparison of AL by MRI in CS group (n = 4, **P < 0.01 compared with the control group, **P < 0.01 compared with SI group); c Rat retinal imaging by optical coherence tomography (OCT) across the center of the optic nerve head (ONH). The measured data of the ganglion cell complex (GCC) was 94.54, 65.07, and 51.10 pixels in the control, SI, and CS groups after 28 days of operation, respectively; d Comparison of the thickness of GCC in the CS group (n = 6, **P < 0.01 compared with the control group, **P < 0.01 compared with SI group). Scale bar = 100 μm; e Representative images of organelles in retinal ganglion cells (RGCs) and photoreceptor cells in the peripheral retina of ocular hypertension (OHT) models. Endoplasmic reticulum (ER) and mitochondria in RGCs were observed by transmission electron microscope (TEM) after 28 days of operation (arrows)