Fig. 3

IκBα overexpression reversed the effects of TRIM46 on cell growth inhibition, hyper permeability and inflammatory response in HRCECs. a Protein level of IκBα in HRCECs transfected with oeTRIM46 or Vector. b–g HRCECs transfected with oeTRIM46 or Vector with overexpression of IκBα (oeIκBα). Cell proliferation and cell cycle was detected with CCK-8 assay (b) and flow cytometry (c), respectively. d Transmembrane electrical resistance and (e) FITC-dextran leak assay was conducted after the cells grew to confluence. f Western blot was used to detect the protein levels of Occludin and ZO-1. g The levels of TNF-α, IL-1β and IL-6 in supernatant were determined by ELISA. h Western blot was used to detect the protein level of NF-κB in the nucleus and plasma and of IκBα. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. CCK, cell counting kit; ELISA, enzyme-linked immunosorbent assay; FITC, fluorescein isothiocyanate; HRCECs, human retinal capillary endothelial cells; NF-κB, nuclear factor kappa B; TRIM, tripartite motif