Fig. 7
From: Transcription factor Foxp1 is essential for the induction of choroidal neovascularization
Foxp1 KO does not change the apparent vascular bed. Adult control and Foxp1 KO mice were perfused with fluorescein isothiocyanate dextran (FITC-dextran) through the left ventricle. The melanin bleaching procedure using H2O2 was applied to the whole eyeballs. The retina and RPE-choroid-sclera complex were separated and flat-mounted on the slides (n = 4 mice). a Bright-field views of the RPE-choroid-sclera layers with the pigment bleached. Scale bar = 200 μm. b Fluorescent images of the FITC-dextran from the RPE-choroid-sclera complex. No apparent shrunken area or accumulation of fluorescence was detected. OD: optic disc. Scale bar = 200 μm. c Fluorescent micrographs of the retinal capillary. Scale bar = 200 μm. d High-magnification pictures showing the deep plexus layer of retinal capillaries. Scale bar = 50 μm