Fig. 6

RNA-seq analysis of retina tissues from FD-Inj-Br and FD-Inj-Wa groups and IHC verification. a The expression of myopia-related genes in the FDM-Inj-Br group and FDM-Inj-Wa group. In the FDM-Inj-Br group, Col1a1 and Mmp2 expression levels were significantly increased, and Slc39a5 expression level was significantly decreased (*P < 0.05). b Distribution of differentially expressed genes in the FDM-Inj-Br group and FDM-Inj-Wa group. Compared with the FDM-Inj-Wa group, 216 genes were upregulated in the FDM-Inj-Br group, and 78 genes were downregulated. c The functional enrichment of genes with increased expression after intravitreal injection of brimonidine was obtained through GO analysis. The results showed that among the top 10 most significant items, the items related to tissue regeneration were as follows: vasculogenesis, blood vessel development, and vasculature development. d Overlap with the myopia-related gene list. The genes significantly upregulated in FD-Inj-Br compared with FD-Inj-Wa and the gene sets involved in the biological process of tissue regeneration in the GSD and Mouse Genome Informatics (MGI) databases were compared by Venn diagram. e Immunohistochemical staining and analysis of retinal tissue in the FD-Inj-Br group and FD-Inj-Wa group. f The expression level of the Mmp2 protein in the retinal tissues of the brimonidine group was significantly increased compared with that in the water group (yellow triangle indicates Mmp2-positive signal), but there was no significant difference in the expression level of the Col1a1 protein