Fig. 5

MiR-184 downregulates CARM1, LASP1, and CDC25A expression in HCECs. a. Putative miR-184 binding sites in CARM1, LASP1, and CDC25A 3′-UTR. Specific binding sites are shown in bracket pairs next to their gene symbol. Alignment between miR-184 and the predicted miR-184 targets. Their conserved 7-bp seed sequence is shown for miR-184:mRNA pairing. b. HEK-293 cells were co-transfected with the expression vectors that were constructed – a pRL-SV40 reporter plasmid and miR-184 mimic or NC. After 24 h, relative luciferase activities were measured. Results are indicated as relative luciferase activity normalized to Renilla luciferase activity (n = 3/group). c. CARM1, LASP1, and CDC25A protein levels determined by Western Blot analysis decreased in HCECs after transfection with miR-184. Ectopic miR-184 expression significantly reduced the levels of CARM1, LASP1, and CDC25A in HCECs compared to the NC. The internal control was GAPDH. d. Densitometric Western Blot analysis quantifying the protein expressions of CARM1, LASP1, and CDC25A was performed (n = 3/group)