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Fig. 5 | Eye and Vision

Fig. 5

From: Faulty homocysteine recycling in diabetic retinopathy

Fig. 5

DNA methylation of homocysteine metabolizing enzymes. Isolated genomic DNA from retinal microvessels were utilized to quantify 5mC levels at the promoters of (a) CBS and (b) MTHFR using methylated DNA immunoprecipitation and IgG as an antibody control (^). c Dnmt1 gene transcripts were measured by q-RTPCR using β-actin as a housekeeping gene. Each measurement was made in duplicates in 5–7 samples in each group, and the data are represented as mean ± SD. *p < 0.05 vs. nondiabetic donors

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Eye and Vision

ISSN: 2326-0254