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Table 1 Enriched Gene Ontology terms and KEGG pathways identified for proteins similarly expressed in F-AME and C-AME (between 0.5- and 2-fold) of 3 donor amnion samples

From: A cellular and proteomic approach to assess proteins extracted from cryopreserved human amnion in the cultivation of corneal stromal keratocytes for stromal cell therapy

 

Enrichment scores

Biological pathways

Proteins (UniProt Accession no.)

p

(A) Enriched GO terms (DAVID)

 1

6.55

GO:0038095~Fc-epsilon receptor signalling pathway

P20618, P25789, P25786, P28070, P01700, O14818, P63208, P60900, P49721, P49720, P63098, P0DOY3

2.8E-6*

 2

6.55

GO:0038061~NIK/NF-kappaB signalling

P20618, P25789, P25786, P28070, O14818, P63208, P60900, P49721, P49720

2.3E-6*

 3

6.55

GO:0043488~regulation of mRNA stability

P20618, P27695, P25789, P25786, P28070, O14818, Q92945, P60900, P49721, P49720

1.9E-6*

 4

3.87

GO:0000209~protein polyubiquitination

P20618, P25789, P25786, P28070, O14818, P63208, P60900, P49721, P49720

4.6E-04*

 5

3.63

GO:0098609~cell-cell adhesion

P30041, O60437, P62820, P46109, Q92817, Q15907, P43487

0.02*

 6

3.12

GO:0000398~mRNA splicing, via spliceosome

P52597, Q08170, P22626, P14866, Q16629, Q86U42

0.19

(B) KEGG pathways (DAVID)

 1

6.55

hsa03050: Proteasome

P20618, P25789, P25786, P28070, O14818, P60900, P49721, P49720

1E-5*

 2

3.43

hsa04610: Complement and coagulation cascades

P01008, P02746, P01009, P00488

0.043*

 3

3.426

hsa03010: Ribosome

P42766, P46779, P39019, Q969Q0, P62979, P62899, P62917, P62266, Q02878, P42677

0.082

 4

3.113

hsa03040: Spliceosome

P26368, O14776, P51991, O75533, Q13435, P62312, O43143, P09012, P08621, Q6P2Q9

0.072

  1. GO = gene ontology, KEGG = kyoto encyclopedia of genes and genomes, F-AME = fresh human amnion extract, C-AME = cryopreserved human amnion extract, DAVID = database for annotation, visualization and integrated discovery
  2. The biological events were ranked using enrichment scores. *Adjusted Benjamini p < 0.05 represents statistical significance