Molecular marker | Antibody | Source | Working dilution |
---|---|---|---|
Calbindin D-28K | Rabbit polyclonal | Swant (CB-38a) | 1:500 |
Recoverin | Rabbit polyclonal | Proteintech (10073-1-AP) | 1:500 |
ACE2 | Rabbit polyclonal | Abcam (ab15348) | 1:200 |
CRALBP | Mouse polyclonal | Abcam (ab15051) | 1:200 |
GFAP | Mouse polyclonal | Sigma (#G3893) | 1:200 |
Iba-1 | Rabbit polyclonal | WAKO (#019–19,741) | 1:1000 retinal sections, 1:200 wholemounts retinas |
Collagen type IV | Goat polyclonal | Millipore (AB769) | 1:1000 retinal sections, 1:500 wholemounts retinas |
AF555 | Rabbit, mouse | ThermoFisher Scientific (A31572, A31570) | 1:100 |
AF488 | Rabbit, mouse | ThermoFisher Scientific (A210206, A21201) | 1:100 |
AF633 | Goat | ThermoFisher Scientific (A21082) | 1:100 |
TO-PRO (NucRed Dead 647) | – | Invitrogen (R37113) | 1:1 |