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Table 1 Primary and secondary antibodies used in this study

From: Microglia activation and neuronal alterations in retinas from COVID-19 patients: correlation with clinical parameters

Molecular marker

Antibody

Source

Working dilution

Calbindin D-28K

Rabbit polyclonal

Swant (CB-38a)

1:500

Recoverin

Rabbit polyclonal

Proteintech (10073-1-AP)

1:500

ACE2

Rabbit polyclonal

Abcam (ab15348)

1:200

CRALBP

Mouse polyclonal

Abcam (ab15051)

1:200

GFAP

Mouse polyclonal

Sigma (#G3893)

1:200

Iba-1

Rabbit polyclonal

WAKO (#019–19,741)

1:1000 retinal sections, 1:200 wholemounts retinas

Collagen type IV

Goat polyclonal

Millipore (AB769)

1:1000 retinal sections, 1:500 wholemounts retinas

AF555

Rabbit, mouse

ThermoFisher Scientific (A31572, A31570)

1:100

AF488

Rabbit, mouse

ThermoFisher Scientific (A210206, A21201)

1:100

AF633

Goat

ThermoFisher Scientific (A21082)

1:100

TO-PRO (NucRed Dead 647)

Invitrogen (R37113)

1:1

  1. ACE2 = angiotensin-converting enzyme 2; CRALBP = cellular retinaldehyde–binding protein; GFAP = glial fibrillary acidic protein; Iba-1 = ionized calcium binding adaptor molecule 1; AF = Alexa Fluor