Fig. 4

Representative Western blots, quantification of αSMA in different periods and regions of scleras in models. a Representative Western blots of αSMA in central and periphery scleras 4 weeks postoperatively in three groups; b Representative Western blots of αSMA 1, 2, 4 weeks postoperatively in two experimental groups; c Expression of αSMA in central and periphery scleras 4 weeks post-operatively in circumlimbal suture (CS) group [n = 6, **P < 0.01 compared with control or sclerosant injection (SI) group in central scleras]; d Expression of αSMA 1, 2, 4 weeks post operatively in CS group (n = 6, **P < 0.01 compared with SI group after 4 weeks of operation, **P < 0.01 in CS group after 4 weeks of operation compared with 2 weeks). The central sclera corresponded to the posterior sclera according to the division of the eyeball wall [19]