Fig. 3
The collagen volume fraction (CVF) of posterior eyeball wall and collagen crimp of peripheral sclera. a–c The CVF was measured by MASSON staining after 28 days of operation in circumlimbal suture (CS) group. Scale bar = 100 μm; d Comparison of the CVF in posterior segment of eyeball wall in CS group [n = 6, *P < 0.05 compared with sclerosant injection (SI) group, *P < 0.05 compared with control group]; e–g Representative transmission electron microscope (TEM) images of the outer peripheral sclera after 28 days of operation, showing lamellar structure formed by collagen fibril bundles in transverse (Tc) section (n = 4). The peripheral scleral collagen was dissolved and absent in the CS group (arrows). Scale bar = 1 μm; h–j Representative TEM images of the outer peripheral sclera after 28 days of operation, showing lamellar structure formed by collagen fibril bundles in longitudinal (Lc) sections (n = 4). The peripheral scleral collagen crimp was found in the CS group (arrows). Scale bar = 1 μm