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Fig. 2 | Eye and Vision

Fig. 2

From: TRIM46 aggravated high glucose-induced hyper permeability and inflammatory response in human retinal capillary endothelial cells by promoting IκBα ubiquitination

Fig. 2

TRIM46 interacts with IκBα and promotes IκBα ubiquitination in HRCECs. a Interference or overexpression of TRIM46 for 24 h was followed by treatment with HG (25 mM). Control osmotic pressure was controlled with a normal glucose concentration of 5.5 mM and mannitol. Western blot detected the protein level of NF-κB in the nucleus and plasma and of IκBα. b, c The interaction between TRIM46 and IκBα detected by Co-IP assay. d After interference or overexpression of TRIM46 in HRCECs, the protein and mRNA levels of IκBα were detected by Western and qRT-PCR. e HRCECs were transfected with oeTRIM46 or Vector and the expression of IκBα was detected by Western blot after treatment with 10 μmol/L MG132 or Vehicle (DMSO) for 4 h. f The ubiquitination of IκBα was detected by IP assay. CCK, cell counting kit; HG, high glucose; HRCECs, human retinal capillary endothelial cells; NF-κB, nuclear factor kappa B; qRT-PCR, quantitative real-time PCR; TRIM, tripartite motif

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Eye and Vision

ISSN: 2326-0254