Fig. 1

TRIM46 enhanced HG-induced cell growth inhibition, hyper permeability and pro-inflammatory factors secretion in HRCECs. a 100% confluent HRCECs were treated with different concentrations of glucose (10, 15, 25 mM). Normal glucose concentration (5.5 mM) and mannitol were used to control the osmotic pressure of control cells. Western blot and qRT-PCR were used to detect the expression of TRIM46 after 24 h of treatment. b–f In HRCECs, TRIM46 was interfered with or overexpressed for 24 h, and then treated with HG (25 mM). Control osmotic pressure was controlled with normal glucose concentration of 5.5 mM and mannitol. Proliferation and cell cycle was detected with the CCK-8 assay (b) and flow cytometry (c), respectively. Cell permeability was analyzed by transmembrane electrical resistance (TEER) (d) and FITC-dextran leak assay were performed after the cells grew to confluence (e). f Western blot show TRIM46, Occludin and ZO-1 expression levels. g The levels of TNF-α, IL-1β and IL-6 in the supernatant was determined by ELISA. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. CCK, cell counting kit; ELISA, enzyme-linked immunosorbent assay; FITC, fluorescein isothiocyanate; HG, high glucose; HRCECs, human retinal capillary endothelial cells; qRT-PCR, quantitative real-time PCR; TRIM, tripartite motif