Fig. 4
From: Complement C3 deficiency alleviates alkylation-induced retinal degeneration in mice
C3 knockout (KO) protects against a reduction in photoreceptor cell number. a Representative images for immunofluorescence staining of rhodopsin in retinal samples before and 1, 3, and 7 days after MMS injection. b Semi-quantification of the rhodopsin staining intensity in each group using ImageJ. C3−/− KO showed significantly more rhodopsin-positive cells than wild-type (WT) mice. c The rhodopsin mRNA level in MMS-treated mouse retinal tissue was evaluated by real-time qPCR and is expressed as the fold change after normalization to mβ-actin level (n = 6, ***P < 0.001 compared with control). MMS, methyl methane sulfonate; GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer