Fig. 4
From: Transcription factor Foxp1 is essential for the induction of choroidal neovascularization
Foxp1 KO reduces vascular endothelial growth factor A (VEGF-A) expression but not pigment epithelium derived factor (PEDF). a Representative Western blot of VEGF-A in RPE-choroid lysates of Foxp1-KO (Foxp1fl/flSix3-Cre+) and control mice (Foxp1fl/flSix3-Cre−). α-tubulin was used as the loading control. VEGF-A protein was lower in Foxp1-KO mice than in the control. b–c Relative densitometry of VEGF-A and PEDF normalized to α-tubulin. ns: no significant difference. d–e Immunostaining of VEGF (green) and PEDF (red) in the KO and control from laser-treated mice on day 7. VEGF staining was reduced around choroidal neovascularization (CNV) in the KO mice compared to the control. Scale bar = 100 μm. RPE, retinal pigment epithelium; GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; SC, sclera; *: P < 0.05, n = 4, Student’s t-test