Fig. 2

Choroidal neovascularization (CNV) is accompanied by increased Foxp1 expression. a Representative spectral domain optical coherence tomography (SD-OCT) B-scan images at the indicated times following laser treatment. Arrows point to the location of CNV in the choroid. Scale bar = 50 μm. b CNV thickness was calculated from the SD-OCT images. c Cryostat sections from the eyes burned by the laser were immunostained at day 7 with the antibodies against CD31 (white) and Foxp1 (red). Scale bar = 100 μm. d Magnified images of the square box in (c). Arrows depict the colocalization of Foxp1 and the endothelial marker CD31. Nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; CH, choroid; SC: sclera. ***: P < 0.001, n = 3 in each group. Statistical analysis was performed by one-way ANOVA followed by Tukey’s post-hoc test