Fig. 7
From: SUV39H1 regulates corneal epithelial wound healing via H3K9me3-mediated repression of p27
SUV39H1 represses p27 expression level during corneal epithelial wound healing (CEWH) in mice. a Western blotting analysis shows SUV39H1, p27, and p21 protein levels in corneal epithelium in wound healing (WH) and control (CT) groups at 48 h after injury (n = 3/group). b Densitometric quantification of SUV39H1, p27, and p21 protein expression in CEWH was performed (n = 3/group). c RT-qPCR analysis shows SUV39H1, p27, and p21 mRNA levels in corneal epithelium of WH and CT groups at 48 h after injury (n = 4/group). d Graphical illustration of the design of primers spanning different regions of the mouse p27 promoter. e Chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR) was used to analyze the H3K9me3 marks at the p27 promoter site in the corneal epithelium in WH and CT groups at 48 h after injury from three independent replicates. Results are presented as percentage (%) of input signal