Fig. 6
From: SUV39H1 regulates corneal epithelial wound healing via H3K9me3-mediated repression of p27
SUV39H1 knockdown upregulates p27 and p21 expression via reducing H3K9me3 marks at their promoters. a Western blotting was performed to measure protein levels of cell cycle regulators at 48 h after siRNA transfection in human corneal epithelial cells (HCECs) (n = 3/group). b Histogram of the quantified protein expression of cell cycle regulators in HCECs after siRNA transfection (n = 3/group). GAPDH is a normalized control. c RT-qPCR was used to assess SUV39H1, p27, and p21 expression levels at 48 h after siRNA transfection in HCECs (n = 3/group). d Diagrammatic representation provides the design of primers spanning different regions of human p27 and p21 promoters. e Chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR) was used to analyze the H3K9me3 marks at the p27 and p21 promoters at 48 h after siRNA transfection in HCECs (n = 3/group). Results are expressed as percentage (%) of input signal