Fig. 3

Knockdown of SUV39H1 suppresses human corneal epithelial cell (HCEC) proliferation and induces G1 cell cycle arrest. a RT-qPCR measured the mRNA levels of SUV39H1/2 in HCECs at 48 h after transfection with irrelevant negative control (NC) or SUV39H1 and/or SUV39H2 siRNA (n = 3/group). b Western blotting examined the protein levels of SUV39H1/2 in HCECs at 48 h after NC or SUV39H1 and/or SUV39H2 siRNA transfection (n = 3/group). c MTS assay assessed cell proliferation at different time points after transfection of NC or SUV39H1 and/or SUV39H2 siRNA (n = 6/group). d Representative images of EdU staining in HCECs at 48 h after transfection with NC or SUV39H1 and/or SUV39H2 siRNA. Red: EdU; blue: Hoechst 33342; scale bar is 50 μm. e Histogram of proliferating HCECs provides values expressed as a percentage of EdU positive cells in NC or SUV39H1 and/or SUV39H2 siRNA transfected HCECs (n = 3/group). f Flow cytometry from three independent replicates determined the cell cycle distribution of NC or SUV39H1 and/or SUV39H2 siRNA transfected HCECs at 24 h after release from the second thymidine block. Representative results are shown