Fig. 2

Using J-OCT to obtain the corneal thickness profile in a normal eye. a The original image of the central horizontal meridian of one normal eye. A specular reflection of the corneal apex ensured that the OCT scanning probe was aligned perpendicular to the cornea. b The image processed by the J-OCT. The red line corresponds to the outer surface of the epithelium; the pink line corresponds to the inner surface of the epithelium; the green line corresponds to the inner surface of the Bowman’s layer; and the yellow line corresponds to the endothelial layer. Stromal thickness was measured as the perpendicular distance between the inner surface of Bowman’s layer and the endothelial layer, which include the stroma, Descemet’s membrane, and the endothelium biologically. The latter two layers were too thin to be noted in the image