Table 2 Primer sequences for RT-qPCR
From: The critical role of m6A methylation in the pathogenesis of Graves' ophthalmopathy
Gene | Forward Primer (5′ - 3′) | Reverse Primer (5′ - 3′) |
|---|---|---|
WTAP | TTGTAATGCGACTAGCAACCAA | GCTGGGTCTACCATTGTTGATCT |
METTL3 | AGATGGGGTAGAAAGCCTCCT | TGGTCAGCATAGGTTACAAGAGT |
METTL14 | GAACACAGAGCTTAAATCCCCA | TGTCAGCTAAACCTACATCCCTG |
FTO | AACACCAGGCTCTTTACGGTC | TGTCCGTTGTAGGATGAACCC |
ALKBH5 | AGTTCCAGTTCAAGCCTATTCG | TGAGCACAGTCACGCTTCC |
HNRNPA2B1 | ATTGATGGGAGAGTAGTTGAGCC | AATTCCGCCAACAAACAGCTT |
ELF3 | GGCCGATGACTTGGTACTGAC | GCTTGCGTCGTACTTGTTCTTC |
YTHDF1 | ATACCTCACCACCTACGGACA | GTGCTGATAGATGTTGTTCCCC |
YTHDF2 | GTTGGTAGCGGGTCCATTACT | GGTCTTCAGTTTAGGTTGCTGT |
YTHDF3 | TCAGAGTAACAGCTATCCACCA | GGTTGTCAGATATGGCATAGGCT |
YTHDC1 | AACTGGTTTCTAAGCCACTGAGC | GGAGGCACTACTTGATAGACGA |
YTHDC2 | GGTATCCCCTGCCGTATATTTTG | CTTTCCCGTCTCTCTGCGG |
GAPDH | GGAGCGAGATCCCTCCAAAAT | GGCTGTTGTCATACTTCTCATGG |