Table 1 Expression primers
Gene | GeneBank Accession No. | Sequences | |
|---|---|---|---|
Oligonucleotide primers | |||
1 | hTGFβ1 | NM_000660.7 | F: GACTGCGGATCTCTGTGTCATT |
R: CAGTAGTGTTCCCCACTGGTC | |||
2 | hTGFβ3 | NM_003239.4 | F: CTTCCAGATCCTTCGGCCAG |
R: ATCAAAGGACAGCCACTCGG | |||
3 | hTSG6 | NM_007115.4 | F: AAGGATGGGGATTCAAGGAT |
R: GCTTGTATTTGCCAGACCGT | |||
4 | mARG1 | NM_031508.1 | F: GATTATCGGAGCGCCTTTCT |
R: CCACACTGACTCTTCCATTCTT | |||
5 | mCol3a1 | NM_009930.2 | F: CGTAAGCACTGGTGGACAGA |
R: CGGCTGGAAAGAAGTCTGAG | |||
6 | mFN1 | NM010233.2 | F: TACTCGAGCCCTGAGGATGG |
R: GCAAGGCAACCACACTGACT | |||
7 | mHAS2 | BC080281 | F: CCAAGGTTCTGCTTCCTCAC |
R: CTCTCCATACGGGGAGAGTC | |||
8 | miNOS | NM_001313922.1 | F: TCACCTTCGAGGGCAGCCGA |
R: TCCGTGGCAAAGCGAGCCAG | |||
9 | mSPARC | XM_030245730.1 | F: ATGAGGGCCTGGATCTTCTT |
R: CACGGTTTCCTCCTCCACTA | |||
10 | mSMA | NM_009696.3 | F: TGTGCTGGACTCTGGAGATG |
R: GAAGGAATAGCCACGCTCAG | |||
11 | mTNC | NM_035737.2 | F: GACTGCCCTGGGAACTGTAA |
R: CATAGCCTTCGAAGCACACA | |||
TaqMan assay IDs | |||
7 | hTGFβ1 | NM_000660.7 | Hs99999918_m1 |
8 | hTGFβ3 | NM_003239.4 | Hs04398989_m1 |
9 | hGAPDH | NM_001256799.3 | Hs01120607_g1 |
10 | mCD80 | NM_009855.2 | Mm00711660_m1 |
11 | mLcn2 | NM_032517.1 | Mm01324470_m1 |
12 | mPLAU | NM_032899.1 | Mm01149438_m1 |
13 | mPpbp | NM_023785.3 | Mm00470163_m1 |
14 | mSpp1 | NM_001204203 | Mm00436767_m1 |
15 | mG6pdx | NM_032034.3 | Mm00656735_g1 |