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Fig. 4 | Eye and Vision

Fig. 4

From: The anti-scarring effect of corneal stromal stem cell therapy is mediated by transforming growth factor β3

Fig. 4

TGFβ3 knockdown in human CSSC. a Human CSSC were transfected with hTGFβ3 siRNA or scrambled sequences. After co-culture with RAW cells under M1 phase in a Transwell system for 48 h, naïve hCSSC and hCSSC transfected with scrambled sequences showed similar TGFβ3 upregulation, whereas cells with TGFβ3 siRNA transfection showed reduced expression (decreased by 70–84%) of hTGFβ3. Similar results were obtained in two separate experiments. b The reduced hTGFβ3 expression was specific for si-TGFβ3 transfection, while there was no changes for scrambled sequences. The expression of hTGFβ1 remained unchanged with TGFβ3 knockdown in CSSC. Mean and SD are calculated from triplicate experiments

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Eye and Vision

ISSN: 2326-0254