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Fig. 1 | Eye and Vision

Fig. 1

From: The anti-scarring effect of corneal stromal stem cell therapy is mediated by transforming growth factor β3

Fig. 1

TGFβ3 upregulation in human CSSC under M1 pro-inflammatory condition. a Mouse macrophage RAW264.7 at M0 phase was treated with LPS and IFNγ for 48 h to M1 pro-inflammatory phase. The cells were differentiated and became adherent with spindle shape appearance. When treated with IL4, they were induced to M2 proliferating phase mimicking anti-inflammatory condition. Scale bar: 80 μm. b M1 and M2 marker expression. Mouse iNOS (miNOS) was upregulated in M1 phase and mouse ARG1 (mARG1) was induced under M2 condition. Co-culture of hCSSC with mouse RAW cells in different phases showed human TSG6 (hTSG6) upregulation under M1 phase. c Expression of hTGFβ1 and hTGFβ3 under co-culture of hCSSC with mouse RAW cells in different phases. Significant upregulation of hTGFβ3 in M1, but not in M0 and M2 conditions. Values represent the mean of triplicate experiments. * P < 0.05, paired Student’s t-test

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Eye and Vision

ISSN: 2326-0254