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Fig. 6 | Eye and Vision

Fig. 6

From: MicroRNA-184 negatively regulates corneal epithelial wound healing via targeting CDC25A, CARM1, and LASP1

Fig. 6

The effects of CARM1 and LASP1 downregulation on human corneal epithelial cell (HCEC) proliferation and migration. a. Reductions in CARM1 and LASP1 protein expression levels caused by gene silencing obtained with siRNAs were confirmed by Western Blot analysis. b. Densitometric Western Blot analysis showing the difference in protein levels between the negative control (NC)- and the siRNA-transfected groups (n = 3/group). c. CARM1 knockdown induced G2/M phase arrest in HCECs. HCECs collected at 48 h after transfection with either siCARM1 or NC were stained with PI and analyzed with flow cytometry. d. Knockdown of CARM1 or LASP1 inhibited the migration of HCECs. Representative photos of the scratched regions were taken by phase-contrast microscopy. e. The percentage of wound closure (%) was quantified by normalizing the healing area to the initial wound area in NC- and the siRNA-transfected groups (n = 3/group)

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Eye and Vision

ISSN: 2326-0254