Fig. 3

Ectopic miR-184 transfection suppresses human corneal epithelial cell (HCEC) proliferation and migration. a. MTS cell proliferation assay was performed at 48 h after transfection with either a miR-184 mimic or negative control (NC). The miR-184 transfected cell number was significantly decreased as compared with the NC (n = 3/group). b. G2/M phase arrest in the HCEC cell cycle was induced by ectopic miR-184 transfection. HCECs gathered at 48 h after transfection were then stained with PI and analyzed with flow cytometry. c. miR-184 transfection reduced HCEC migration. HCECs were transfected with miR-184 or NC for 24 h, and scratches were then made in the HCEC cultures to perform the wound-healing assay. Following culturing for either 0 or 24 h in serum-free DMEM/F12, representative photos of the scratched regions were taken by phase-contrast microscopy. d. The percentage of wound closure (%) was quantified by normalizing the healing area to the initial wound area (n = 3/group)