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Fig. 2 | Eye and Vision

Fig. 2

From: Correction to: Faulty homocysteine recycling in diabetic retinopathy

Fig. 2

Homocysteine metabolizing machinery in diabetic retinopathy. Retinal microvessels were employed to determine CBS (a) gene transcripts by q-RTPCR, (b) protein expression by Western blotting, using β-actin as a housekeeping gene and loading protein, respectively, and (c) enzyme activity by measuring fluorescence at 368 nm excitation and 460 nm emission wavelengths. Values obtained from nondiabetic controls are considered as 100%. Gene transcripts of (d) MTHFR and (e) CSE were quantified by q-RTPCR using β-actin as a housekeeping gene. Data are represented as mean ± SD, obtained from retinal microvessels from 6 to 8 nondiabetic and 7–8 diabetic retinopathy donors. *p < 0.05 vs. nondiabetic donors

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