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Fig. 6 | Eye and Vision

Fig. 6

From: Faulty homocysteine recycling in diabetic retinopathy

Fig. 6

Effect of homocysteine supplementation on oxidative stress and DNA methylation machinery in isolated human retinal endothelial cells. HRECs, incubated in a medium containing homocysteine were analyzed for (a) CBS gene transcripts by q-RTPCR, (b) ROS levels by DCFH-DA method, and (c) CYTB and (d) DNMT1 gene transcripts by q-RTPCR. β-actin was employed as a housekeeping gene for all q-RTPCR measurements. Results are represented as mean ± SD from 3 to 4 different cell preparations, with each measurement made in duplicates. Cont and + Hcy = cells incubated in normal incubation medium and normal incubation medium containing homocysteine, respectively. *p < 0.05 vs Cont

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