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Fig. 3 | Eye and Vision

Fig. 3

From: Identification, quantification and age-related changes of human trabecular meshwork stem cells

Fig. 3

Representative confocal images of isolated TM cell cytosmears immunostained for (a) ABCG2 (FITC-green) and p75 (Alexa 633-red) and (b) AnkG (FITC-green) and p75 (Alexa 633-red), counterstained with propidium iodide (PI-blue). The cells with mean pixel intensity 188 ± 24 (Mean ± SD) were graded high positive (++), 125 ± 42 positive (+) and 53 ± 28 as negative for ABCG2. The expression of p75 and AnkG was graded either positive (+) or negative (−). Nuclear and cytoplasmic area were measured by marking the region of interest (ROI) around each nucleus and cell on the PI/bright field overlay image. The cells in the upper right (UR, first row) quadrant of the scatter plot were highly positive for ABCG2 with high N/C ratio and these were designated as stem cells. These UR cells also expressed p75 and AnkG. Lower right (LR, second row) quadrant cells had high N/C ratio but low ABCG2 expression. The white arrow head indicates a cell in the upper left (UL, third row) quadrant having high ABCG2 expression but low N/C ratio and lower left (LL, fourth row) quadrant cells had low ABCG2 expression and low N/C ratio. +/+ − cell positive for both AnkG and p75; −/− cell negative for AnkG and p75. Scale bar 10 μm

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