A cellular and proteomic approach to assess proteins extracted from cryopreserved human amnion in the cultivation of corneal stromal keratocytes for stromal cell therapy

Fenner, Beau J.; Yusoff, Nur Zahirah B. M.; Fuest, Matthias; Zhou, Lei; Bandeira, Francisco; Cajucom-Uy, Howard Y.; Tan, H. K.; Mehta, Jodhbir S.; Yam, Gary H. F.

doi:10.1186/s40662-019-0155-0

Eye and Vision

Table 2 Top 40 proteins with comparable expression (< 2 and > 0.5 fold) between C-AME and F-AME in all amnion samples

From: A cellular and proteomic approach to assess proteins extracted from cryopreserved human amnion in the cultivation of corneal stromal keratocytes for stromal cell therapy

No.	UniProt Accession No.	Protein	Protein symbol	Mean Abundance*	Mean fold changes**
1	P02768	Serum albumin	ALB	457	0.89
2	Q09666	Neuroblast differentiation AHNAK	AHNAK	435	0.89
3	O75369	Filamin-B	FLNB	408	1.03
4	Q99715	Collagen 12A1	COL12A1	407	1.09
5	Q8IVF2	AHNAK Nucleoprotein 2	AHNAK2	242	0.82
6	P12111	Collagen 6A3	COL6A3	207	1.5
7	Q13813	Spectrin α chain	SPTAN1	205	1.64
8	Q15149	Plectin	PLEC	178	0.86
9	P02452	Collagen 1A1	COL1A1	173	0.63
10	P01023	α2-macroglobulin	A2M	172	0.8
11	P68871	Haemoglobin subunit β	HBB	169	0.59
12	P08670	Vimentin	VIM	158	1.99
13	P02787	Serotransferrin	TF	157	0.88
14	P02545	Prelamin-A/C	LMNA	146	1.37
15	A8K2U0	α2-macroglobulin-like protein 1	A2ML1	145	0.75
16	P01024	Complement C3	C3	142	1.08
17	P07355	Annexin A2	ANXA2	124	0.72
18	P06396	Gelsolin	GSN	122	0.91
19	P01861	Immunoglobulin heavy constant γ4	IGHG4	120	0.6
20	P14618	Pyruvate kinase	PKM	113	0.99
21	P01859	Immunoglobulin heavy constant γ2	IGHG2	109	0.56
22	P24821	Tenascin	TNC	108	0.62
23	P02461	Collagen 3A1	COL3A1	108	0.99
24	P30101	Protein disulfide-isomerase A3	PDIA3	98	0.56
25	P11142	Heat shock cognate 71 kDa protein	HSPA8	94	0.89
26	P15311	Ezrin	EZR	94	1.9
27	P07585	Decorin	DCN	92	0.87
28	P11021	78 kDa glucose-regulated protein	HSPA5	92	1.08
29	P63104	14–3-3 protein zeta/delta	YWHAZ	88	0.6
30	P0C0L5	Complement C4-B	C4B	83	0.88
31	P0C0L4	Complement C4-A	C4A	80	0.94
32	P98160	Basement membrane-specific heparan sulphate proteoglycan core protein	HSPG2	80	1.09
33	P05787	Keratin 8	KRT8	77	1.76
34	P08603	Complement factor H	CFH	73	0.64
35	Q14697	Neutral α-glucosidase AB	GANAB	72	1.55
36	P26038	Moesin	MSN	71	0.74
37	Q16555	Dihydropyrimidinase-related protein 2	DPYSL2	71	1.41
38	Q05707	Collagen 14a1	COL14A1	69	1.99
39	P13667	Protein disulfide-isomerase A4	PDIA4	68	0.94
40	P02788	Lactotransferrin	LTF	68	0.72

F-AME = fresh human amnion extract, C-AME = cryopreserved human amnion extract
*The protein list was ranked by mean abundance. **Mean fold changes from all 3 amnion samples

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ISSN: 2326-0254

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