Fig. 4From: A cellular and proteomic approach to assess proteins extracted from cryopreserved human amnion in the cultivation of corneal stromal keratocytes for stromal cell therapyPrimary human keratocyte cultures in ERI medium supplemented with F-AME or C-AME. a Phase contrast micrographs showing CSK morphology after 5 passages in culture. Primary CSKs were prepared using donor corneal stromal tissue, HC778 and HC787. b Cell viability by calcein AM staining and cell quantification. Data are presented as mean and standard deviation for 4 primary CSK cultures. c Cell proliferation by EdU incorporation assay and cell quantification (n = 4 primary CSK cultures). d Wide-field spinning disk confocal laser microscopy for CSKs immunostained for ALDH3A1 (green fluorescence) and CD34 (red fluorescence). e Cell quantitation for the percentage of CSKs expressing ALDH3A1 and CD34 by confocal immunofluorescence in 4 primary CSK cultures. f Mean percentages of CSKs expressing ALDH3A1 and CD34 by immunostaining. Error bars: standard deviation. Scale bars: 300 μm (a), 500 μm (d)Back to article page