Fig. 1
From: Bowman layer transplantation in the treatment of keratoconus
Surgical views of a Bowman layer (BL) graft preparation. A donor globe is mounted on a globe holder or a donor corneo-scleral rim is placed on an artificial anterior chamber with its epithelial side up. Corneal epithelial cells should be removed, after which, (a) just within the limbal area a superficial incision can be made over 360° with a 30G needle. (b) A peripheral donor BL edge is then lifted from the underlying anterior stroma using a single tip of a McPherson forceps. (c-e) Subsequently, by grasping the BL edge with the McPherson forceps via gentle slow movements in a circular manner, the entire BL is carefully peeled away to free the tissue from its underlying attachments. After preparation, the BL graft is evaluated and can be trephined if needed. (f) The BL graft tends roll into a single or double roll due to tissue elasticity