Table 1 Antibodies used in the study
From: Toll-like receptor 2-mediated NF-kappa B pathway activation in ocular surface epithelial cells
Antibody | Species & Type | Manufacturer | Catalogue Number | Dilution factor |
|---|---|---|---|---|
IκB-α | rabbit polyclonal | Santa Cruz | SC-371 | 1:1000 |
NF-κB p50 | goat polyclonal | Santa Cruz | SC-1190 | 1:20 for IF, 1:1000 for WB |
NF-κB p65 | rabbit polyclonal | Santa Cruz | SC-109 | 1:20 for IF 1:1000 for WB |
phosphor-IκB-α (Ser32/36) (5A5) | mouse monoclonal | Cell Signalling Technology | #9246 | 1:5000 |
Anti-Rabbit HRP conjugated secondary antibody | goat polyclonal | Santa Cruz | SC-2030 | 1:5000 |
Anti-mouse HRP conjugated secondary antibody | goat polyclonal | Santa Cruz | SC-2005 | 1:5000 |
Anti-goat HRP conjugated secondary antibody | rabbit polyclonal | Santa Cruz | SC-2768 | 1:5000 |
Anti-Rabbit Alexa Fluor 488 conjugated secondary antibody | goat polyclonal | Sigma-Aldrich | SAB4600234 | 1:400 |
Anti-goat Alexa Fluor 488 conjugated secondary antibody | donkey polyclonal | Abcam | Ab150129 | 1:1000 |