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Table 1 Antibodies used in the study

From: Toll-like receptor 2-mediated NF-kappa B pathway activation in ocular surface epithelial cells

Antibody Species & Type Manufacturer Catalogue Number Dilution factor
IκB-α rabbit polyclonal Santa Cruz SC-371 1:1000
NF-κB p50 goat polyclonal Santa Cruz SC-1190 1:20 for IF, 1:1000 for WB
NF-κB p65 rabbit polyclonal Santa Cruz SC-109 1:20 for IF 1:1000 for WB
phosphor-IκB-α (Ser32/36) (5A5) mouse monoclonal Cell Signalling Technology #9246 1:5000
Anti-Rabbit HRP conjugated secondary antibody goat polyclonal Santa Cruz SC-2030 1:5000
Anti-mouse HRP conjugated secondary antibody goat polyclonal Santa Cruz SC-2005 1:5000
Anti-goat HRP conjugated secondary antibody rabbit polyclonal Santa Cruz SC-2768 1:5000
Anti-Rabbit Alexa Fluor 488 conjugated secondary antibody goat polyclonal Sigma-Aldrich SAB4600234 1:400
Anti-goat Alexa Fluor 488 conjugated secondary antibody donkey polyclonal Abcam Ab150129 1:1000