Fig. 2

Effects of picrotoxin and picrotoxin plus SCH 23390 on the ERG waves a, b Time course of the effects of picrotoxin (PT) and picrotoxin plus SCH 23390 (PT + SCH) on the amplitudes of the ERG b- and d-waves. The results of different groups are indicated with open symbols in control experiments; hemi filled symbols in PT experiments and filled symbols in PT + SCH experiments. The amplitudes of the ERG waves were normalized to the values obtained just prior to picrotoxin application (10th minute from the beginning of the experiment). The times when the perfusion was switched to PT and PT + SCH are indicated by arrows. Mean values ± SEM are shown. c Original ERG records (b- and d-waves) obtained in the PT group during the perfusion with Ringer solution in the control period (10th minute from the beginning of the experiment - upper row) and during the perfusion with 50 μM picrotoxin: 37th minute (middle row) and 48th minute (bottom row) from the beginning of the experiment. The stimulus onset and offset are indicated below the traces. Calibration: time – 0.2 s; amplitude – 50 μV. d Original ERG records (b- and d-wave), obtained in the PT + SCH group during the perfusion with Ringer solution in the control period (10th minute from the beginning of the experiment - upper row), 50 μM picrotoxin (37th minute from the beginning of the experiment - middle row) and 50 μM picrotoxin + 75 μM SCH 23390 (48th minute from the beginning of the experiment - bottom row). The stimulus onset and offset are indicated below the traces. Calibration: time – 0.2 s; amplitude – 50 μV