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Fig. 8 | Eye and Vision

Fig. 8

From: Corneal surgery in keratoconus: which type, which technique, which outcomes?

Fig. 8

Reconstruction of corneal stroma. a: Hematoxylin-eosin staining of a rabbit cornea with an implanted graft of decellularized human corneal stroma with h-ADASC colonization: hypocellular band of ECM without vessels or any inflammatory sign (magnification 200X); b: Human cells labeled with CM-DiI around and inside the implant that express (c) human keratocan (human adult keratocyte specific marker; magnification 400X), confirming the presence of living human cells inside the corneal stroma and their differentiation into human keratocytes (arrows); d: Phase-contrast photomicrographs showing a morphologically unaltered corneal stroma (magnification 400X); e: The graft remains totally transparent after 12 weeks of follow-up (magnification 2X) (arrows point to the slightly visible edge of the graft). Abbreviations: Epi: epithelium; Str: stroma; Lam: Lamina

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