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Table 3 PCR product and restriction product sizes expected in diagnostic test

From: Development and validation of a novel PCR-RFLP based method for the detection of 3 primary mitochondrial mutations in Leber's hereditary optic neuropathy patients

1

2

3

4

5

Uncut PCR products

MaeIII Non-mutated

MaeIII 3460A

MaeIII 11778A

MaeIII 14484C

333

307

279

307

307

236

236

236

236

205

164

164

164

135

164

  

28

29

31

 

26

26

26

26

  1. Column 1: PCR product sizes (bp) generated in the multiplex PCR. Column 2: Size of MaeIII restriction products obtained from non-mutated DNA samples. Column 3: Size of MaeIII restriction products obtained from 3460A samples. Column 4: Size of MaeIII restriction products obtained from 11778A samples. Column 5: Size of MaeIII restriction products obtained from 14484C samples
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Eye and Vision

ISSN: 2326-0254