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Table 3 PCR product and restriction product sizes expected in diagnostic test

From: Development and validation of a novel PCR-RFLP based method for the detection of 3 primary mitochondrial mutations in Leber's hereditary optic neuropathy patients

1 2 3 4 5
Uncut PCR products MaeIII Non-mutated MaeIII 3460A MaeIII 11778A MaeIII 14484C
333 307 279 307 307
236 236 236 236 205
164 164 164 135 164
   28 29 31
  26 26 26 26
  1. Column 1: PCR product sizes (bp) generated in the multiplex PCR. Column 2: Size of MaeIII restriction products obtained from non-mutated DNA samples. Column 3: Size of MaeIII restriction products obtained from 3460A samples. Column 4: Size of MaeIII restriction products obtained from 11778A samples. Column 5: Size of MaeIII restriction products obtained from 14484C samples
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Eye and Vision

ISSN: 2326-0254