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Table 2 Primer sequences for multiplex PCR-RFLP

From: Development and validation of a novel PCR-RFLP based method for the detection of 3 primary mitochondrial mutations in Leber's hereditary optic neuropathy patients

Mutation Forward Primer Reverse Primer Product Size
G3460A 5'-CCCCTACGGGCTACTACAACCCTTCGCTGtC 5'-GATAGTAGAATGATGGCTAG 333 bp
G11778A 5'-AGCAAACTCAAACTACGAACG 5'-TTACTAGCACAGAGAGTTCTC 164 bp
T14484C 5'-AATAGCCATCGCTGTAGTATATCCAAAGACAgtCA 5'-GTGCGAGAATAATGATGTATGC 236 bp
  1. Lowercase letters indicate alterations introduced to the forward primers, which when in combination with the mutation, generate a MaeIII site. PCR product sizes are indicated