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Table 2 Primer sequences for multiplex PCR-RFLP

From: Development and validation of a novel PCR-RFLP based method for the detection of 3 primary mitochondrial mutations in Leber's hereditary optic neuropathy patients

Mutation

Forward Primer

Reverse Primer

Product Size

G3460A

5'-CCCCTACGGGCTACTACAACCCTTCGCTGtC

5'-GATAGTAGAATGATGGCTAG

333 bp

G11778A

5'-AGCAAACTCAAACTACGAACG

5'-TTACTAGCACAGAGAGTTCTC

164 bp

T14484C

5'-AATAGCCATCGCTGTAGTATATCCAAAGACAgtCA

5'-GTGCGAGAATAATGATGTATGC

236 bp

  1. Lowercase letters indicate alterations introduced to the forward primers, which when in combination with the mutation, generate a MaeIII site. PCR product sizes are indicated
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Eye and Vision

ISSN: 2326-0254