Plasmid | Nucleotide Positions | Normal Mix | 3460A mix | 11778A mix | 14484C mix |
|---|
3460G (N) | 3275 – 4272 | X | | X | X |
3460A (M) | 3275 – 4272 | | X | | |
11778G (N) | 11580 – 12118 | X | X | | X |
11778A (M) | 11580 – 12118 | | | X | |
14484 T (N) | 14449 – 15022 | X | X | X | |
14484C (M) | 14449 – 15022 | | | | X |
- The table presents nucleotide positions, based on the reference sequence NC_012920.1, included in the cloned mitochondrial plasmids. 3460G indicates the normal G nucleotide at position 3460 and 3460A indicates the mutated A at position 3460; similarly, for 11778 and 14484 plasmids. The synthetic diagnostic control mixes are generated by mixing the indicated plasmids at 1.5 ng/μl. For a diagnostic test, 1 μl of a diagnostic control mix is used
